ABSTRACT
The present study was aimed at exploring the native wild isolates of Penicillium chrysogenum series in terms of their penicillin production potential. Apart from the standard medium, the efforts were made to utilize suitable agro-industrial wastes for the maximum yield of penicillin. Two series of P. chrysogenum were isolated from local sources and named as P. chrysogenum series UAF R1 and P. chrysogenum series UAF R2. The native series were found to possess better penicillin production potential than the already reported series of P. chrysogenum. However, P. chrysogenum series UAF R1 was found to be the best candidate for high yield of penicillin starting at 100 hour as compared to P. chrysogenum series UAF R2 which produced the highest yield of penicillin at 150 hours for a shorter period of time. Addition of Corn Steep Liquor (CSL) to the fermentation medium resulted in the production of 1.20g/L penicillin by P. chrysogenum series UAF R1 and P. chrysogenum series UAF R2. The fermentation medium in which Sugar Cane Bagasse (SCB) was replaced with CSL resulted in the highest yield of penicillin (1.92g/L) by both native series of P. chrysogenum. The penicillin production was increased by 62.5% in medium with SCB as compared to that with CSL. The penicillin yield of medium containing lactose and phenyl acetate was higher than that of control medium. Overall results revealed that P. chrysogenum series UAF R1 and P. chrysogenum series UAF R2 may be recommended for better yield of natural penicillin and this efficiency may be further enhanced by utilizing SCB as substrate in the growth medium.
Subject(s)
Antifungal Agents/analysis , Antifungal Agents/isolation & purification , Fungicides, Industrial/analysis , Lactose/analysis , Penicillins/isolation & purification , Penicillium chrysogenum/isolation & purification , Saccharum , Zea mays , Enzyme Activation , Methods , Plant StructuresABSTRACT
Se comprobó el efecto inductor mutagénico de la luz uv, obteniendo mutantes auxotróficos y morfológicos de salmonella typhimurium. El aislamiento de mutantes auxotróficos mediante la técnica de réplica en placa mejora con el uso de penicilina mediante el método de Hollyday, para la identificación de los requerimientos específicos de cada mutante, se caracterizó 3 mutantes auxotróficos de 24 mutantes escogidos al azar. De 24 mutantes auxotróficos, el 30 por ciento resultó ser metronina dependientes, y 25 por ciento revirtieron la características de mutantes auxotróficos.
Subject(s)
Salmonella typhimurium/chemistry , Salmonella typhimurium/classification , Salmonella typhimurium/growth & development , Salmonella typhimurium/isolation & purification , Salmonella typhimurium/virology , Penicillins/isolation & purification , Replica Techniques/trends , Replica TechniquesABSTRACT
To isolate high penicllin yielding mutants, the efficacy of [NTG] on Penicillium chrysogenum under various conditions was studied. Conidia were treated with various concentrations of N-MethyI-N-Nitro-N-Nitrosoguanidine [NTG] for 30 minutes. Complete inactivation of spores was observed at 1x10-3M. A maximum of 4% auxotrophs were obtained at 5x10-5M. Using 5x10-4M [NTG] as a given concentration, conidia were treated over different time intervals, the lethality was increased to 99% in 40 minutes. The frequency of auxotrophs increased linearly with time intervals. The optimum condition for the isolation of high penicillin yielding mutants is at a concentration of 5x10-4M [NTG] with a 16 to 24 minutes